This investigation was carried out during 2013 to 2015 seasons in tissue culture laboratory of Horticulture Department, Agriculture Faculty, Mansoura University, to make comparssion between shoot tips and epicotyls explants  in vitro propagation of five Citrus rootstocks namely, Sour orange (Citrus  aurantium, L.), Volkamer lemon (Citrus  volkmeriana, Ten.). Cleopatra mandarin (Citrus reshni, Blanco), Rangpur  lime and Troyer citrange . Our results showed that, epicotyl of Rangur rootstock, Sour orange and Volkamariana which cultured on MS medium containing BAP+Kin (BAP at 1 mg/L, Kin at 1.5 mg/L) gave distinct significant increase of length of shoots/plantlet from epicotyl explants comparing with another tested treatment in this study. Also, explants of epicotyl that took from Volkamariana, Sour orange and Rangpur on MS medium supplemented with IBA+NAA(IBA at 2 mg/L, NAA at 2mg/L) in vitro had significant increase of length of roots/explant comparing with other two citrus rootstocks in this respect.