The present investigation was conducted during 2012, 2013 and 2014 seasons on 10-year-old Superior grapevines cultivar grown in a sandy soil, planted at 2 m within rows and 3 m between rows and irrigated using drip irrigation system at a private vineyard located at El-Khatatba, Menoufiya governorate, Egypt. The obtained data was discussed in details for the last 2 seasons only. The main objective of this investigation was to study the effect of fulvic acid foliar application 9 ml/liter /vine, either alone or in combination with micro-elements (Fe SO4. 7H2O at 0.36 g + Zn SO4.7H2O at 0.18 g + MnSO4. H2O at 0.18 g) and bio-fertilizers (Trichoderma viride + Trichoderma harzianum + Serratia sp. + Pseudomonas fluorescens + Bacillus polymyxa) at 7.14 ml/liter/vine (104/ml per fungi and 109/ml per bacteria viable cell) on vegetative growth, total chlorophyll content, yield, physical and chemical characteristics of cluster and quality of berries, total carbohydrates, total N and C/N ratio in canes of Superior seedless grapevines. Results revealed that the combined treatment of fulvic acid, micro-elements plus bio-fertilizers induced indispensable positive effects for enhancing vegetative growth, total chlorophyll content, yield, physical and chemical characteristics of cluster and quality of berries, total carbohydrates, total N and C/N ratio in canes of Superior grapevines. A stronger effect may probably support the hypothesis of that humic substances have different effects on plant adequately supplied with nutrients, in sustainable or organic viticulture for production of organic products, which can be a noteworthy alternative to synthesized chemicals fertilizers to produce a healthy product-free from their toxic residues and fit for export. Foliar spray applications of these products can have prospects for optimal economical use in terms minimizing the cost of production and in turn increased the income of vineyards.