Two field experiments were conducted during 2013 and 2014 growing seasons at Nubaria Region, Alexandria Governorate, Egypt in order to study the integrated effect of mineral nitrogen and biofertilizer (85 kg N/fed as recommended dose (control), 65 kg N/fed + 600 g Cerealin as biofertilizer and 45 kg N/fed + 600 g Cerealin) on three sweet sorghum varieties i.e., Sorgo, Brandes and Planter.  Results showed that Planter variety recorded the highest values of morphological characters i.e. stalk height, stalk diameter, leaf area, stripped, and juice yields as well as chlorophyll a and b concentrations compared with Sorgo and Brandes in both seasons. On the other hand, in both seasons syrup yield was significantly increased in Brands variety compared with other two varieties. In both seasons Planter variety gave the highest values of physiological characters i.e., sucrose%, pH value, total soluble solids%, purity% and extracted juice%, while, Sorgo variety recorded the highest value in reducing sugar. The treatment of 65 kg N/fed + 600 g Cerealin gave the greatest concentrations of chlorophyll a and b in both seasons also gave the greatest values of stalk height, diameter and leaf area in the first season only, while, stripped stalks and juice yields recorded the highest values in both seasons. In the first season there was no significant difference in juice yield at the rate of 85 kg N/fed compared with 65 kg N/fed 600 g Cerealin, while, in the second season both treatments did not significantly differed in stripped stalks yield. Nitrogete fertilizer at rate of 65 kg N/fed + 600 g Cerealin gave the highest values of sucrose%, purity% and juice extraction% in both seasons. The interactions between Planter variety and 65 kg N/fed + 600 g Cerealin gave the greatest values in stripped stalks, juice and syrup yields followed by Brandes and Sorgo in both seasons.