This investigation was carried out at the experimental farm of EL-Serw Agriculture Research Station and Seed Technology Research Unit Mansoura, Agricultural Research Center from 2012 to 2015 year to study the effect of cuts number (without cut (control), one cut and two cuts) and harvesting dates (25, 32, 39, and 46 days after 50% flowering) on fresh and seed yield, of Sudan grass as well as, seed quality after different storage periods. The main results can summarized as follows: Left Sudan grass plants without cut (control) and harvesting after 39 day from 50% flowering gave the highest values of plant height, straw fresh and dry weight kg/ plot, 1000-seed weight and seed yield / plot. Two cut treatments associated with the highest values of total fresh and dry forage yield/plot. Seed produced from the fourth harvest date without cutting gave the best values of seed germination percentage and seedling vigor traits followed by one cut then two cuts under different storage periods conditions. Increasing storage periods from six to 18 months led to the reduction in seed quality traits. So, sorghum Sudan grass plants one- cut before seed production could be considered the most suitable treatment to produce high yield and seed quality.