This study was conducted during two seasons 2014 and 2015 to evaluate the effects of spraying natural moringa leaf extract, silicon and their combination on vine vigor, yield and fruit quality of "Flame seedless" (Vitis vinifera L.) grapevine grown in a private vineyard located at El-Beheira Governorate. Seven treatments were done, control (spraying with water), silicon at 0.1%, moringa extract at 2%, and silicon at 0.1% plus moringa extract at 2%. All treatments were sprayed in two ways (2 times only and every 2 weeks) starting at 15-20 cm of shoot length. The obtained results revealed that the suggested treatments were effective in stimulating different growth parameters, yield, and fruit quality[G1]  parameters. Moringa leaves extract at 2% combined with silicon at 0.1% every two weeks significantly enhanced vegetative growth parameters in terms of leaf area, total chlorophyll, average shoot length, cane internodes length, [G2] and their diameter, and removed pruning weight, leaf nutrients content as N, P, K, Fe, Zn and Mn. Also cluster physical quality parameters in terms of weight, length, width, berry physical parameters as weight and volume of 100 berries, firmness, berry removal force, berry length and diameter consequently and juice chemicals in terms of SSC, SSC/acid ratio, anthocyanins and total sugars contents are also increased. Sequently, it increased the total yield per vine and reduced titratable acidity.