The present work was conducted during 2015 and 2016 seasons at the Tropical Farm, Aswan Botanical Garden, Agric. Res. Inst. to study the possibility of propagated Chrysophyllum oliviforme L.vegetatively.  Semi- hardwood cuttings from juvenile (3 years old) and mature (30 years old) C. oliviforme trees were used to test their prospect to form the adventitious roots. The growth media consisted of 2:1 peat: perlite (v: v) ratio and the base of cuttings were soaked for 24 h. in different IBA concentrations (0, 1000, 2000, 4000 and 5000 ppm).  The obtained results showed significant differences in the root and shoot development of cuttings with a higher rooting parameters forthe young than mature trees. Using the high hormone concentrations (4000- 5000 ppm) increased the rooting percentage and growth parameters compared to the low concentrations (1000- 3000 ppm). However, the anatomy of cuttings postulate that sectors of mature trees had a thicker stripe of xylem vessels and additional cell layers of sclerenchyma than sectors from juvenile trees. Also, cambium was more differentiated with high activity in young trees than in the mature one. You can say that the presence of a continuous sclerenchyma layer can work as a physiological partition to the emerging root and increase with the aging of the tree.