Two field experiments were conducted in a loam soil at the private Farm of El-GamaliaDakahlia Governorate, Egypt during the summer seasons of 2014 and 2015 to study the effect of the various combinations among organic manures, bio and mineral fertilization on the production of safe and economic potato tubers spunta cultivar. Twenty-four treatments were arranged in split – split plot design with 3 replicates, which were the simple possible combination between two sources of organic manure [Farmyard manure (FYM) and compost] as main plots, treatments of mineral fertilization (50, 75, and 100% NPK from recommended doses) as sub plots and three sources of bio fertilization (EM, yeast extract and mixture of multi strains) as sub-sub plot. Thus, the total numbers of every experiment were 72 experimental units. The obtained results indicated that;the mean values of, fresh, dry weights of tubers /plant , number of tuber and total yield of potato ton fed^-1 as well as cholorophyll content after 70 days in leaves, Nitrite (No₂-N), Nitrate (No₃-N), reducing sugars percentages, non-reducing sugars percentages and total sugar percentageswere significantly affected due to adding FYM (20 ton fed^-1) and compost (15 ton fed^-1) during both seasons of the experimentation. The highest mean values of traits were recorded with adding compost. As for, the mean values of parameters under study were significantly increased with increasing NPK from 50 up to 75% of the recommended dose during both seasons, then decreased with adding 100%. In the same way the highest significant values of the aforementioned parameters were recorded with spraying plants by mixture of bio- fertilization(Azotobacterchroccoccum (AZ), Bacillus megatherium (PSB) and Bacillus circulans (KSB)) (2 ml/L.) followed by spraying EM (2 ml/plant).The highest mean values were recorded with 15 ton fed^-1. compost and inoculation with mixture and 75% NPK RD.