Cultivation of Catharanthus roseus L.under saline water irrigation as a simulation of water salinity in Sinai, conditions were investigated in response to amino acid treatments. Two pot experiments were conducted at the Desert Research Center, Mataria, Cairo Egypt, and its experimental farm at El-Maghara Experimental Station in North Sinai Governorate, Egypt during the two successive seasons of 2014 & 2015.Established seedlings were irrigated with freshly prepared saline water using NaCl at 2000, 4000 and 8000 ppm plus tap water as a control treatment. All treatments received amino acid treatments as a foliar spray at 0, 0.5 and 1 g/liter using Amino zeid 42/85 a commercial product containing 85% total amino acids and 42.3 % highly concentrated free amino acids.Results indicated that damage of salinity was severed in a higher concentration of saline water irrigation treatment to reach the death of plants irrigated with 8000 ppm after the first cut. Alkaloids production in different plant parts was increased within saline water concentration increase levels. Meanwhile, most of the growth traits were enhanced using amino acid treatments under other saline water irrigation conditions. Combining irrigation with amino acids treatments at 1 g/l resulted in enhancing of growth traits under 2000 and 4000 ppm treatment. Although amino acids enhanced growth under moderate salinity condition, results indicate that Catharanthus roseus plants still need susceptible to salinity damage. From our observation the use of amino acids for enhancing C. roseus salinity tolerance still more research using higher concentration or other methods of amino acids application