Two field experiments were carried out during the two successive summer seasons of 2014 and 2015 at the experimental farm of the Environmental Studies And Research Institute, University of Sadat City to study the effect of some chemical, organic and bio fertilizers on seed yield and its components as well as rust disease infection of Kafer EL-Shaikh cawpea cultivar. seven treatments were used in addition to control treatment.. Results indicate the treatment of 50% NPK +Rhizobia + Homic + Microbin and 50% NPK + Rhizobia + Homic were the best among all tested treatments where both of them gave an average high-yield and some of its components compared with the other treatments in both seasonsAs for rust disease, the low percentage of infection has always been linked to the presence of humic acid and in some cases with the compost, on the other hand, the ratio was high percentage of infection in most cases linked to the existence of chemical fertilizer and sometimes the presence of rhizobia, or both together. The treatment 50% NPK + Rhizobia +Homic+Microbin was the best while it give the lowest percentage of infection under the four periods in the two seasons. The recommended added rates were as follows: 50% NPK. (16.5kg N+ 30 kg P₂O₅ + 25kg K₂O) +2kg commercial rhizobia + 4L commercial homic +5L commercial microben / feddan.