This study was evaluating the effect of diode laser with power 160 mw and He-Ne laser with power 7 mw on date palm callus.  Diode laser was irradiate Date Palm Callus for 4,8,12 and 16 minutes and 5, 10, 15 and 20 minutes for He-Ne laser respectively. Generally 10 min irradiation in He-Ne and 8 min in diode laser significantly increased the content of total soluble sugars and total indol compared with control. In addition 20 min irradiation in He-Ne and 16 min in diode laser increased the content of total phenols and total soluble amino acids compared with control. DNA analysis and protein Electrophoreses were study the similarity of genetic at different duration of Laser irradiation for date palm callus. DNA analysis showed a higher similarity between 10 min and control treatment ( 93% ) while Lower similarity ( 85% ) between 15 min and control treatment was recorded .