A protocol for indirect micro propagation of Thymus vulgaris was developed using shoots tips explants derived from ex vitro mother plants. Murashige and Skoog (MS) Medium with different concentrations of auxins (NAA, IAA and 2,4-D) alone or in combination with different cytokinins (BA and Kin) were used to induce callus formation. MS medium supplemented with 2,4-D at 2 mg/L in combination with Kin at 1 mg/L produced the highest callus formation percentage of 100 % and significantly counted spectacularly callus fresh weight of 9.56 g. The highest Callus regeneration percentage (100 %) commitment to the highest significant shoots number (23.42 shoots/ explant) were obtained on MS medium provided with BA at 2.5 mg/L. The preferable rooting of shoots was obtained on MS medium half strength augmented with 30 g/L sucrose and NAA at 1.5 mg/L. On this medium, 100 % of the shoots produced roots with a mean number of 19.83 roots per shoot. Shoots with well-developed roots were successfully established in pots filled with mixture of soil: peat moss (1: 1 v/v). The survived plantlets had a survival rate of 98 %.