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41294

Micropropagation of thyme plant (Thymus vulgaris)

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Last updated: 04 Jan 2025

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Abstract

The current research was conducted at Vegetable and Floriculture Department in the experimental station and tissue culture laboratory, Agriculture Faculty, Mansoura University. The objective of this research was to develop a direct plant regeneration of a valuable aromatic and medicinal plant (Thymus vulgaris) by in vitro culture of shoot tips or nodal segments from mature plants on MS medium supplemented with different cytokinin type at different concentrations. Nodal segments were found to be more efficient than shoot tips for Thymus vulgaris shoots regeneration on MS medium (Murashig and Skoog 1962) supplemented with 6-benzyladenine (BA) at 2 mg/L. The best elongation was obtained on MS medium supplemented with BA at 2 mg/L in combination with GA3 at 0.5 mg/L.The best rooting of shoots was obtained on MS medium augmented with α-naphthaleneacetic acid (NAA) at 1.5 mg/L. Regenerated plants were successfully established in pots filled with mixture of soil: peat moss (1: 1 v/v). In vitro Thymus vulgaris plants had a survival rate of 100 %, and showed healthy and uniform growth.

DOI

10.21608/jpp.2017.41294

Authors

First Name

H.

Last Name

El-Banna

MiddleName

Y.

Affiliation

Vegetable & Floriculture Department, Faculty of Agriculture, Mansoura University.

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Volume

8

Article Issue

11

Related Issue

6502

Issue Date

2017-11-01

Receive Date

2019-07-17

Publish Date

2017-11-01

Page Start

1,221

Page End

1,227

Print ISSN

2090-3669

Online ISSN

2090-374X

Article File

JPP_Volume 8_Issue 11_Pages 1221-1227.pdf

PDF . 259.7kB

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https://jpp.journals.ekb.eg/article_41294.html

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https://jpp.journals.ekb.eg/service?article_code=41294

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Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

MainTitle

Micropropagation of thyme plant (Thymus vulgaris)

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Article

Created At

22 Jan 2023