Silicon (Si) is considered as one of the most vital elements for the plant life. Nanoparticles interact with plants causing many changes morphologically and physiologically based on the properties of NPs. Nano silicon dioxide (with diameter 20:40 nm) was synthesized from sodium metasilicate and inclusively characterized. Murashige and Skoog media were supplemented with nano SiO₂ in four levels (0, 50,100 and 150 ppm) in addition to IBA in order to find the interaction mechanism between nanosilica and banana plants under control condition in vitro. The efficiency of Nano SiO₂ was evaluated in terms of its impact on some morphological traits, total chlorophyll content and total phenolic compounds were also assessed. Results showed a positive effect of silicon dioxide nanoparticles on rooting rate and photosynthesis pigments. Overall, application of Silicon in the nano scale was beneficial in improving growth of banana.