This investigation was carried out on female Jojoba (Simmondsia Chinensis, (Link) Schneider) shrubs in a private orchard at Hackstep, Zone, Cairo Governorate to evaluate 15 female shrubs, about 8 years old during two successive seasons of 2014 and 2015. The shrubs growing in sandy soil in 4 lines at distances 3 × 3 meter apart and irrigated with drip irrigation system. Through the rigorous assessment of female selected jojoba trees through the measurements of vegetative growth, floral measurements, productivity and the percentage of oil in their seeds. The study recommends the following: Firstly: the strongest shrubs were No. (1, 2, 5, 9, 10, 12 and 15), secondly: the shrub number No 4 was the earliest one in the flowering date in both first and second seasons. Thirdly: the highest yield recorded for female shrubs No. (5, 10, 12 and 15) in the first season, while in the second one were No. 9 and 12 per the branch. Fourthly: the female shrubs No. (2, 5, 7 and 13) gave the highest oil content in the first season, while in the second one, the female shrub No. 11 was the best. Fifthly:  genetic fingerprint of different species was made using 15 primers and it resulted in 5 positive primer and unaffected 10 then, when using ISSR marker the results showed that there are differences between breeds. It is clear from the foregoing that the shrubs No. 5,4,12, and 11, 13) were the best in the strength of growth, early harvest, production and oil content percentage and can be more vegetative and cultivated. As for a high value of genetic similarity was No. 8 and 6 and also, the highest similarity value (0.875) was found between the strains (T8 and T6) followed by 0.792 between (T5 & T15, T8 & T13 and T11 & T14), respectively. Generally, genetic similarity value was low among the investigated genotypes and the study.