The present investigation aimed to evaluate some Egyptian cotton cultivars i.e.: Giza 86, Giza 94, Giza 87, Giza 88, Giza 92, Giza 93 and Giza 96 under three different locations in Lower Egypt (Delta) during the ‎two successive growing seasons of  2015 and 2016 for yield, seed quality and viability characters. Randomized complete ‎block design with three replications was used at each location. Significant differences for cultivars and locations for all studied characters, except free fatty acid and acid value. The effects of the growing years, interactions between (genotype x seasons), (location x season) and the second order interaction were significant on most studied characters, Also the effect of the interaction between (genotype x locations) was significant for all studied characters, except for seed cotton yield and lint percentage. Giza 94 and Giza 92 surpassed in seed cotton yield, boll weight, lint percentage and seed index. The cultivar Giza 92 gave the highest oil % and germination, while, Giza 96 produced the highest radical length, shoot length and seedling dry weight, while. Giza 88 surpassed all studied cultivars in protein %.Positive significant correlations were found between seed cotton yield and both boll weight and seed index, lint percentage and each (boll weight, seed volume and seed coat), boll weight and seed coat %, seed index and both (seed volume and seedling dry weight), free fatty acid and acid value, protein % and seed coat %, seed volume and seedling dry weight, germination % and both (shoot length and seedling dry weight), shoot length and seedling dry weight.