Tow field experiments were conducted at El-Sirw Agricultural Research Station, Damietta Governorate, Egypt during 2015 and 2016 seasons. The main objectives of this study is to estimate the physiological and biochemical performance of some rice varieties under various salinity levels. The experiments were performed in randomized complete block design with four replicationsfor each salinity level apart and the results were statistically analyzed as split plot design after homogeneity test as combined. Soil survey of the experimental farm was annually done to find the following used salinity levels viz; 2, 6 and 10dSm-1.Giza177, Giza178, Giza179 and Egyptian hybrid one (EHR1) ricevarieties were used. Anti –oxidants, physio- morphological and growth parameters were measured at heading stage as well as grain yield and yield attributing characteristics were measured at harvest. The obtained results indicated that: increasing salinity levels led up to decreased potassium percentage (K %), increased sodium percentage (Na %) and Na+/K+ ratio in rice plant. Furthermore, increment of salinity levels reduced dry matter accumulation (g),relative water content RWC(%), chlorophyll a,b and total chlorophyll (µg ml-1) and, increased a/b ratio and antioxidant system peroxidase (POD)(μmol min-1 g-1 protein) Catalase (CAT)(μmol min-1 g-1 protein),proline (mg g-1 FW).Increasing salinity levels decreased the studied growth characteristics; flag leaf area, chlorophyll content (SPAD value), plant height, panicle weight, panicle length, number of filled grains, 1000-grain weight and grain yield/ha-1but increased unfilled grains per panicle. EHR1gave the highest values of most studied characters followed by Giza179 while, Giza178 came in the third order. Giza177 gave the lowest values of the most studied traits. Egyptian hybrid one under the three salinity levels was distinction compared with other rice varieties in both seasons. Based on current biochemical and physiological traits and yield under different salinity levels, the tested rice varieties could be ranked as follows; EHR1>Giza179>Giza178 regarding their salinity tolerance. Giza 177 was found to be more salt sensitive variety.