Two series field experiments were done for the period of 2014/2015 and 2015/2016 seasons at Abo-SaiedVillage, KafrEl-SheikhCenter, Kafr El-Sheikh Governorate to study the effect of plant population and distribution and nitrogen levels on yield and quality of sugar beet, "CV. Zwan Poly". The studied plant distributions were 25, 30 and 35 cm on both sides of terrace (Mastaba) 80 cm width and 20, 24 and 28 cm on both sides of terrace (Mastaba) 100 cm width, which resulted in three plant populations of 30000, 35000 and 42000 plants/fad. The used design was split-plot with 4 replicates. Results showed that plant population and distribution had significant effects on all studied traits.  Cultivating beet seeds at 35 cm distance between hills on both sides of mastaba 80 cm width (30000 plants/fad) resulting significant increases in number of leaves/plant, foliage fresh weight/plant, plant weight, root weight as well as sucrose percentage, total soluble solids(TSS) and purity percentages. Increasing N-levels from 69 to 92 and 115 kg N/fad significantly increased root weight, root diameter, root length, number of leaves, foliage fresh weight/ plant and plant weight in both years, while it significantly decreased sucrose, TSS and purity percentages in both years. Generally, it could be accomplished that cultivating sugar beet on both sides of  mastaba 80 cm width and 25 cm between hills and fertilizing plants with 115.0 kg N/fad could be recommended for maximizing root yield under the ecological circumstances of this research.