This study was carried out during two successive seasons (2015 & 2016) in a private vineyard farm at Boktares village, Aga, Dakahlia Governorate, Egypt. The experiment was conducted on 12 years old Thompson seedless grapevines cultivar in a clay soil under surface irrigation system. The vines were trained on three vertical wires system and cane-pruned. Seeds of four medicinal plants; fenugreek, anise, black cumin and parsley were planted in rows between the vines rows in the fourth week of September 2014 and 2015 seasons. At the same time different plots were cultivated by different medicinal plants as sole crops to compare growth characters and medicinal plants yield under intercropping conditions with individual cultivation of medicinal plants without intercropping. The aim of this study was to evaluate the efficacy of intercropping of Thompson seedless grapevines with some medicinal on vines nutritional status, yield, berry quality and the microbiological activity of the soil. Obtained results revealed that intercropping of Thompson seedless grapevines with all used medicinal plants increased N, P , K and organic matter (O M) in the soil, which enhanced vines nutritional status, vegetative growth, yield and berry quality. Vines intercropped with fenugreek plant significantly increased shoot length, leaf area and total chlorophyll content in the leaves as well as yield per vine, soluble solids content and total sugar, while reduced the total acidity in berries. In addition,increased total microbial count, dehydrogenase and phosphatase enzymes activity in the rhizosphere.The economical study indicated that intercropping Thompson seedless with fenugreek gave the highest net profit/ feddan followed by intercropping Thompson seedless with black cumin, parsley and anise, respectively.