Ocimum species have a major importance not just as an aromatic or ornamental plant which utilized for many softscape purposes, additionally as a medicinal plant because of its high substances from volatile oils and numerous secondary metabolites such as rosmarinic acid, flavonoids and anthocyanins. An overview of the recent studies conducted with the use of plant tissue culture technique for micropropagation by using the direct way like cell, tissue and organ culture or the indirect one as, callus redifferentiation and somatic embryogenesis were reviewed in this paper, because of the genetic variability could occur during the sexual propagation method (seeds). In the same time, production of the important secondary metabolites from Ocimum species by using different methods such as organs culture, cells suspension, cells elicitation, bioreactors and cell immobilization were also reviewed.