Two pots experimentsin were carried out at the experimental farm of Sakha Agricultural Research Station, North Delta, Egypt, during the two winter seasons of  2014/2015 and 2015/2016 to study  certain botanical characteristics(25) varieties (Betavulgaris L).. The obtained results indicated that:(root length, root diameter, root size, root fresh weight, root dry weight, number of leave, fresh and dry weight leaves)showedvarieties highest values withCharlston, Lamiaa, Nefertitis, Salma and Beta 398 Charlstonwas the better one for leaf and root characters. While, the lowest values were recorded with Cawamera, Milaspoly, DEO32-705, HM16584 and OscarpolyIn addition, it was the found that chlorophylls and macro elements content in the leaf, were recorded highest values  forthe varieties Maximus and Charlston, but, the lowest value were cleared with the varieties Alauda , Cawamera and Milaspoly. At the same time, yield total as well as  (sugar, quality, potassium, sodium, α amino nitrogen and TSS showed the maximum values with Charlston, Beta 394, Lamiaa, Salma, Samba and HM586 compared with,Pleno, HM16584, Cawamera, and Milaspoly which  gave the lowest values. The  anatomical studies of roots showed that diameter of root, thickness vascular  of bundle, layer of paranchyma, diameter of vessels and number of growth rings, , recorded the highest values with Charlston variety compared Cawamera variety.Moreover , it was found apossitive correlation  between root dry weight, fresh and dry weight of leaves, chlorophyll A, phosphorus , sugar %, quality%, sodium content,diameter of xylem vessels and thickness of parenchyma layer and growth rings, these resultspropsed to classified  sugar beet varieties to three groups, the first one include the varieties charlston,  Lamiaa, Nefertitis,  Salma,  Beta 398, Beta 394, Samba and HM586 as earlier varieties .The second onewereMaximus, Steel, Nansy,Mona,Lagon, Mimona, Drena, Glorius, Athospolyand HM16101as medium, and the third onewereCawamera, Milaspoly, DEO32-705, HM16584, Oscarpoly, Alauda,  and Pleno, as later for sowing date and may be useful for understanding the mechanisms of sugar content with dry weight, thickness of parenchyma layer, growth rings of the root and date of sowing and maturity of these varieties under the same condition.