Glycinebetaine is an amino component that accumulates in many plant species under drought stress. In order to evaluate the response of vegetative growth, yield components, quality and anthocyanin content of roselle plants to glycinebetaine foliar application under water stress, a field experiment was performed during the two successive seasons of 2017 and 2018 at the Experimental Farm of Sakha Agricultural Research Station, Kafr El-Sheikh Governorate, Egypt as split plot. The irrigation treatments were 50±5, 65 ± 5 and 80±5%  of available soil moisture depletion were allocated in the main plot while glycinebetaine foliar spray (25 and 50 mM) and distilled water as control were distributed in the sub-plots. Results showed that 80% reduced vegetative growth characters, fruit number, fruit fresh weight, calyces fresh and dry weight, calyces yield per plant and per fed as well as seed yield. Glycinebetaine at 25 and 50 mM enhanced vegetative growth and yield characters, TSS, acidity and total anthocyanin. Irrigation at 65 % combined with 25 mM glycinebetaine recorded the highest branch number, fruit number, fruit fresh weight, calyces fresh and dry weights/plant (g), seed yield per plant and calyces yield /fed, photosynthetic pigments, TSS, acidity % and total anthocyanin content while reduced pH value. Foliar spray of 25mM GB with 65 % of available soil moisture depletion was found to be more beneficial for avoiding the effects of water stress on above-mentioned attributes, which could be a feasible technique for roselle production that increases the yield and quality as compared to the other studied treatments.