The production of siderophores is greater in rhizosphere inhabiting fungi and bacteria. The present work was designed to isolate the rhizosphere inhabiting bacteria and fungi from the rhizosphere of Phaseolus Vulgaris, Pisum sativum, Vicia faba and Alfa alfa. The obtained isolates has been screened for siderophores production using Chrome Azurol Sulfonate assay (CAS). The highest siderophore producer bacterium and fungus has been molecularly identified as Bacillus MG214652 andAspergillus niger MH844535 respectively. They were able to produce 70.4% and 87% units of siderophore respectively. Bacillus MG214652 siderophores was characterized as catecholate type with maximum absorbance at 495 nm. Aspergillus niger MH844535 siderophore was characterized as hydroxymate type with maximum absorbance at 450 nm.