Two field trials were conducted during two successive growing seasons of 2016/2017 and 2017/2018 at Mallawy Agricultural Research Station, Agricultural Research Center, El-Minia Governorate (Middle Egypt) to study the effect of different levels of N-fertilization (160,190 and 220 kg /fed) on yield, yield components and quality of five sugar cane varieties (G.T.54-9(C9), G.2003-47, G.99-103, G.2004-27 and G.2010-7). A split plot design with three replications was used, main plots were represented by five sugar cane varieties (V1= G.T.54-9(C9), V2= G.2003-47, V3= G.99-103, V4= G.2004-27, V5= G.2010-7). The subplots were used for the three nitrogen levels (160, 190 and220 kg N / fed). The results indicated that: 1- Sugar cane varieties and N-fertilization levels exhibit significant effect on all studied traits in both seasons. Promising variety G.99-103 recorded the highest values of stalk hieght, stalk diameter (cm), millable cane ton/fed and sugar yield (ton/fed) in both seasons, while G.T.54-9(C9) variety recorded the highest values of sucrose %, purity% and sugar recovery%, whoever G.2004-27 variety outyielded the highest value of TSS%2- Increasing applied N levels from 160 up to 220 Kg N/fed significantly increased stalk height, stalk diameter(cm), millable cane ton / fed, sugar yield (ton/fed) and TSS (%), while190 Kg N/fed had the highest values of purity% and Sugar recovery%.3- The effect of interaction between sugar cane varieties and nitrogen levels on the studied traits was significant for stalk height in both seasons and purity%, sucrose % and T.S.S. % in plant crop season only. Generally, it could be concluded that under Middle Egypt conditions, it is recommended to inoculate G.99-103 sugar cane varieties with 220 Kg N/fed to maximize the productivity and quality of sugar cane yield.