Catharanthus roseus (L.) G. Donis a potential source of several pharmaceutically materials used in cancer treatment. There is a growing demand for its alkaloids that are biosynthesized naturally in a very low quantity. The current study aimed to enhance plant growth and improved internal structure by using certain elicitors. The elicitors tested were calcium  chloride (CaCl₂) at 4.4 g/ l^-1, sodium nitroprusside (SNP) at 0.149 g/l^-1, salicylic acid (SA) at 0.138 g/l^-1 singly or in combination with yeast extract (YE) at either 5 or 10 g/L^-1. Pot experiments were done at the experimental farm of Agric. Bot. Dep., Mansoura Univ., Egypt; during the two growing seasons of 2015 and 2016. Results indicated that, tested elicitors positively affected on studied growth attributes, and the magnitude of effect was elicitor- dependent. Stem length as well as stem dry weight and number of leaves were increased in response to YE at 5 and 10 g/l^-1, respectively. Under the influence of abiotic elicitors, most growth parameters were increased due to SA and SNP. An additive effects were detected in the combination treatments between either SA or SNP and YE. Stem dry weight was highest in plants treated with SA in combination with YE at 5 g/l^-1. In addition, applied elicitors, generally  increased proportions of the photosynthesizing tissues in the leaf and the conductive tissues in the stem. The data suggested to using a combined treatment of SA with YE at 5 g/l^-1 to increase C. roseus biomass, consequently its yield from alkaloids.