Water deficit consider as one of the abiotic factors controlling growth and limiting wheat yield, through affecting on the physiological and biochemical processes within the plant tissues. Two field experiments were carried out in order to investigate the effect of rates from stress defense substances [Jasmonic acid (JA) and Lithovit (Li)] on growth, physiological traits, chemical constitutes and yield of wheat plants (Gemmeiza 11 cultivar) under three water regimes (100, 75 and 50% ET_c) at Experimental Farm, Faculty of Agriculture, Menoufia University, Shebin El-Kom, Egypt during the two growing winter seasons of 2016/2017 and 2017/2018. Exposing plants to water deficit levels (75% and I 50% ET_c) decreased in all growth characters studied, water relations (total water content, relative water content, osmotic pressure, transpiration rate and plasma membrane integrity), photosynthetic pigments, chemical composition (total carbohydrates, total soluble sugars, N and K) as well as yield and its components (number of spikes/ m², number of grains /spike, spike weight, 1000-grain weight, grain yield /fed and straw yield /fed) in comparison with well-watered plants (Control, 100 ET_c). However, plants grown under severe water stress (I 50% ET_c) recorded highest activities of peroxidase, phenoloxidase and increased proline content.Application of JA and lithovit combinations showed an additive effects on increasing all characters studied except transpiration rate, plasma membrane integrity, activities of peroxidase and phenoloxidase as well as proline content compared to untreated plants. Application rate 10 ppm JA + 5% lithovit surpassed most of the other tested characters studied in both growing seasons. The interactions between water regimes and stress defense substances (JA and Li) were found to be significant for most studied traits. Wheat grown under normal irrigation integrated with application of 10 ppm JA + 5% lithovit generally produced the highest values of growth, photosynthetic pigments as well as yield and its components. Moreover, application of 10 or 20 ppm jasmonic acid + 5% lithovit under water stress conditions led to an increase in all vegetative growth characters, water relations (total water content, relative water content and osmotic pressure), photosynthetic pigments, chemical composition of the leaves (total carbohydrates and total soluble sugars contents, N and K percentages) as well as yield and its components, while caused a significant decrease in transpiration rate, plasma membrane integrity, proline, activities of peroxidase and phenoloxidase enzymes. It could be recommended that application of JA integrated with lithovit (10 ppm JA + 5% Li) under water deficit conditions led to mitigate the adverse effects of drought stress and increased growth and productivity of wheat.