The aim of this study was to evaluate the capability of magnetized field in improving the growth, physiological and biochemical aspects and yield attributes of wheat crop (Triticum aestivum L. cv Giza 168) as one of the important economically strategic crops. Pot experiments were executed in a glass greenhouse belonged to the experimental farm, Faculty of Agriculture, Menoufia University, Shibin El-Kom, Egypt during two winter growing seasons 2015/2016 and 2016/2017. Magnetic treatments were magnetic grains, magnetic water and combined magnetic grains and water in addition to the control group. Vegetative growth of wheat plants was significantly increased by treating with different magnetic treatments compared to the control plants. Application of magnetic treatments caused significant increases in total leaf water content, relative water content, water use efficiency (WUE), membrane permeability and succulence degree, whereas the transpiration rate and leaf water deficit were significantly reduced. Most growth characteristics and WUE were more positively affected by the magnetized water treatment than other magnetic treatments. The concentrations of photosynthetic pigments and growth promoters (IAA, GA and Cytokinins) showed significant increases in the treated wheat plants with all magnetic treatments. A marked increase was observed in the uptake of N, P, K, Ca, Mg, Fe, Cu and Mn, but Na uptake was decreased in wheat plants by the application of magnetic field. Magnetic treatments caused a significant increase in the spike characters, grain and straw yield, and harvest index of wheat plants as well as some chemical constituents in grains compared with the control plants.