This research was carried out at the Mushroom Research Laboratory, EL_Kassain Horticulture Research Station, Ismailia Governorate. The research was carried out for two successive seasons, 2016 and 2017. The object of this experiment was to study the effect of some of substrates (rice straw, wheat straw, bean straw, alfalfa hay and pea straw) on physical the yield and its components, of oyster mushroom (Pleurotus florida) fruit bodies. These parameters were measured during the experiment of Cap diameter (cm), Cap weight (gm), fruit body weight (gm), dry matter, early yield (gm), total yield (gm), NPK contents, carbohydrate content and protein content. The results revealed that, there were significant differences in all characters under study, the use of rice straw has achieved the highest results in most of these characters. With regard to chemical constituents of fruit bodies, using rice straw substrate enhanced the percentages of dry matter, phosphorus, potassium and total carbohydrates. On the other hand, the bena straw, pea straw and alfalfa hay were the highest values in nitrogen and crude protein. In generaly the results indicated that, the suitable substrates used for cultivation and production of oyster mushroom was rice straw of the substrate as compared to other substrates.