An experiment was carried out at the Experimental Farm of Sakha Agricultural Research Station, Kafr El-Sheikh, Egypt during the two successive seasons of 2015 and 2016 to investigate the effect of different systems of phosphorus fertilizer, either alone or in combination with biofertilizer (biofertal)  on growth, yield, yield components and seed quality of four cultivars of soybean. The experimental design was split plot design with four replications, the main plots were devoted to soybean cultivars (Giza 21, Giza 35, Giza 111 and Crawford). While, the sub-plots were occupied with different sources of phosphorus fertilizer ; application of 150 kg super phosphate fed.^-1., 100 kg super phosphate fed.^-1 + biofertal, 100 kg super phosphate fed.^-1 + spraying of super phosphate at 4%, 100kg super phosphate fed.^-1+ biofertal+ spraying of super phosphate at 4%, 50 kg super phosphate fed.^-1+ biofertal, 50 kg super phosphate fed.^-1 + spraying of super phosphate at 4%, 50 kg super phosphate fed.^-1+ biofertal+ spraying of super phosphate at 4%. The results showed marked difference of soybean cultivars were observed, among these cultivars, Giza 121 cv. recorded the highest seed yield followed by Giza 21 and Giza 35 while, the lowest seed yield was recorded with Crawford cv. at the two growing seasons. On the other hand, application of 100 kg P₂O₅ fed^.-1 combined with inoculation with biofertal and foliar spray with 4% P₂O₅ fed.^-1 recorded the highest values of photosynthetic pigments, dry weight plant^-1, plant height, number of pods plant^-1, 100-seed weight, seed yield and seed oil percentage at the two growing seasons. The recommendation of the obtained results is application of 100 kg P₂O₅ fed.^-1 combined with inoculation with biofertal and foliar spray with 4 % P₂O₅ fed.^-1 with soybean Giza 111 cv. under the same conditions in the study.