Two greenhouse experiments were conducted during 2014/ 2015 and 2015/2016 seasons in the Experimental Station Farm, Faculty of Agriculture and Natural Resources, Aswan University, Egypt on a sandy textured soil under unheated plastic houses. The target fertilization program seeks to achieve the best combination of various organic and NPK mineral fertilization that lead to the highest yield and quality of produced sweet pepper plant ''Lama Star F₁ hybrid'' especially during the winter season. Therefore, 12 treatments were arranged in a Split plot layout in complete randomize block design with three replicates. Chicken manure at the rates of (10, 15 and 20m³/fed.) were randomly arranged in the main plots, while NPK mineral fertilization at rates of (0:0:0, 50:30:50, 100:40:80, and 150:50:110 Kg N:P₂O₅:K₂O/fed., respectively, were randomly distributed in the sub-plots. The obtained results demonstrated that the treatment combination of chicken manure at 20 m³/ fed. Plus either 150:50:110 or 100:40:80 Kg. NPK/fed. brought about the highest significant mean values of most studied characters as vegetative growth characters (i.e. plant height, number of leaves/plant, the number of branches /plant and plant fresh weight); yield characters {i.e. No. fruits /plant, average fruit weight (g), early yield/m² (kg) and total yield/m² (kg)};  fruit quality (i.e. TSS %,vitamin C, and non-reducing fruit sugars); chemical analysis characters (viz,a, b and total chlorophyll and N, P, K contents in leaves and fruits ) during both seasons of the study as compared to the other treatments. Based upon, the reported results, it is possible to conclude that, the combination among 20 m³/fed. of chicken manure plus either 150:50:110 or 100:40:80 Kg. NPK/fed. considered as the optimal combination treatment whereas it gave the highest mean values of vegetative growth characters, yield and its components and fruit quality of pepper plants grown under plastic houses conditions at Aswan governorate.