Two field experiments were carried at the Experimental Station Farm, Faculty of Agriculture, Mansoura University, Egypt, during 2014 and 2015 seasons to evaluate impact of Farmyard manure (Without – FYM), mineral (100%, 75% and 50% NPK of recommended doses), bio-fertilizers (Without – Yeast - Seaweed - EM) and their interactions on chemical components, quality and yield of okra (Abelmoschus esculentus L.) cv. White velvet. Data obtained in this study cleared that, fertilizing okra plants with FYM (organic treatments) at rate of 20m3/fed increased significantly all measured parameters, i.e. chlorophyll a, b and total, nitrogen, phosphorus and potassium percentages in leaves, crude protein %, total carbohydrates %, crude fibers %, vitamin C, beta carotene, nitrate concentration and total yield/fed compared with control treatment (without) except crude fiber in the second season. However, fertilizing okra plants with 75% NPK from recommended doses gave the highest values of all studied parameters in the first season except nitrate and yield. Meanwhile, the superiority of all parameters mentioned previously in the second season was obtained from 100% NPK treatment except chlorophylls content. Moreover, treated plants with bio-fertilizers increased significantly all studied characters compared with untreated plants, except nitrate content. The interaction treatment among FYM at the rate of 20 m3/fed, 75% NPK from recommended doses and EM (2ml/l) addition gave the highest values of quality and yield with significant differences in both seasons of study. Therefore, this treatment could be recommended for improving okra production and decrease the amount of mineral fertilizer under similar circumstances of this study.