This work aimed to study the effect of incorporating chia seeds flour with corn starch into pudding blends in different ratios ( 1.5:4.5, 3:3, 4.5:1.5 and 6:0 (g/g) per 100 g milk), whereas corn starch (6 g/100 g milk) was used as a control sample. Functional properties of raw materials, physicochemical properties, protein quality, textural properties and sensory properties of prepared pudding samples were evaluated. The incorporation of chia seeds flour resulted in higher functional properties. Additionally, the protein, fat, ash and crude fiber content of pudding increased by 1.3, 1.4, 1.2, and 11.8 times in blend P4 (6:0 (g/g) chia seeds flour per 100 g milk) compared to the control blend (P). Moreover, the sum of essential amino acids and the sum of  non-essential amino acids content were increased from 1.23 to 2.06 (g/100 g sample), and from 2.17 to 4.03 (g/100 g sample), respectively by increasing the chia seeds flour level. Thereby, increasing chia seeds flour level in pudding formula caused increasing values of indicators of protein quality. For textural properties, the cohesiveness increased from 0.48 to 0.68, whereas the other texture parameters demonstrated the opposite behavior by increasing the chia seeds flour level. Sensory evaluation resulted that the blend formed with 3: 3(g/g) chia seeds flour: corn starch (P2) was nearer to those of the control blend in terms of overall acceptability. The study recommends that chia seeds flour could be used as a new way to improve the functional properties and nutritional value of pudding.