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Improving the Efficiency of the Traditional Method of Camel Chymosin Extraction Using Ultrasound and a Freeze-Drying Process

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Last updated: 04 Jan 2025

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Abstract

Many problems are associated with the traditional extracting methods of camel chymosin; they are costly, time-consuming, and have low concentrations of chymosin. This study aimed to increase the efficiency of the traditional methods of camel chymosin production. Two experiments were carried out; the first experiment was implemented using dried camel abomasum as a powder and strips (4-7mm), and exposed to ultrasound exposure time at (20, 40, and 80 min). The chymosin extracted using the dried powder with ultrasound exposure time at 80 min treatments caused a higher significant increase in milk clotting activity and lower proteolytic activity compared to the strips and control treatments. The second experiment was implemented using the freeze-dried process for both chymosin extracted from the strips and powder treatments, which was selected as a higher in total clotting activity and chymosin yield. Upon comparison to the traditional samples, the freeze-dried treatments caused a higher significant increase in specific milk-clotting time and MCA/PA ratio by approximately 4.2 fold and 24.0 fold, respectively. Moreover, the freeze-dried process caused a significant increase in chymosin yield, curd viscosity, firmness, and reduced the syneresis of milk curd. Furthermore, the results confirmed that the viscosimetric measurement can be used as a new indicator to predict the cutting time of camel milk curd. The use of ultrasound with the freeze-dried process can be recommended as an appropriate treatment for the extraction of camel chymosin. 

DOI

10.21608/jfds.2022.174356.1079

Keywords

Camel Chymosin, Ultrasound, freeze-drying, Clotting activity, proteolytic activity

Authors

First Name

A. H.

Last Name

Ibrahim

MiddleName

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Affiliation

Dairy Technology and Production unit - Animal and Poultry Breeding Department- Desert Research Center. Egypt

Email

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City

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Orcid

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First Name

El-sayed

Last Name

Abdeen

MiddleName

Mohamed

Affiliation

Desert research center animal and poultry department dairy science and technology unit

Email

saidabdeen7@gmail.com

City

Cairo

Orcid

ORCID iD https://orc

Volume

13

Article Issue

11

Related Issue

38031

Issue Date

2022-11-01

Receive Date

2022-11-04

Publish Date

2022-11-01

Page Start

165

Page End

173

Print ISSN

2090-3650

Online ISSN

2090-3731

Link

https://jfds.journals.ekb.eg/article_272522.html

Detail API

https://jfds.journals.ekb.eg/service?article_code=272522

Order

2

Type

Original Article

Type Code

886

Publication Type

Journal

Publication Title

Journal of Food and Dairy Sciences

Publication Link

https://jfds.journals.ekb.eg/

MainTitle

Improving the Efficiency of the Traditional Method of Camel Chymosin Extraction Using Ultrasound and a Freeze-Drying Process

Details

Type

Article

Created At

22 Jan 2023