The antimycotoxigenic of Brevibacterium linens (B. linens) against Aspergillus
flavus (A. flavus) in solid and liquid media was studied. Aflatoxins B1, B₂, G₁ and G2
were detected in all tested media using TLC technique and quantitatively determined
by HPLC. The obtained data revealed that B. linens reduced the growth of A. flavus
when the two microorganisms were inoculated on nutrient agar medium at the same
time (zero time) or when B. linens was grown for one day before A. flavus growth.
While, complete inhibition of A. flavus growth was occurred in 2 and 3 days old culture
of B. linens. Also, complete inhibition of aflatoxins production was occurred in B. linens
culture at Zerotime and up to 3 days old. Agitation during B. linens growth on nutrient broth medium is an important
factor to prevent the fungal growth and imeresed production of the antifungal
compound(s). The use of 100% of B.linens culture of 3'days old as a growth medium
for A. flavus inhibited the fungal growth and sporulation. The results indecated that B. linens has potential effect may be use as
biological control against mold growth and mycotoxins production in foods and dairy
products.