The present study aims to explore the role of cell wall pectic polysaccharides of prickly pear peel on some biological activities of rats. This was achieved by altering the structure of cell wall pectic polysaccharides either by physical means (fiber-pectin) or by enzymatically degradation (EPP). Data showed that prickly pear peel represented 40.5 % of the fruit weight. It contained 16.61% alcohol insoluble solids, 12.20% total soluble solids, 7.80 % reducing sugars and 2.27% non-reducing sugars. Biological  results ascertained that the rats fed on various fractions of prickly pear peel fiber, such as fiber – pectin (FP), alcohol insoluble solids (AIS), and enzymatically treated prickly pear peel (EPP), showed a reduction in the body weight gain, serum glucose, cholesterol and triglycerides. Histopathological  experiment showed that feeding rats FP and AIS attained an improvement in liver tissues than the rats fed on hyperlipidemic diet. Statistical analysis ascertained that no significant differences in relative weight of liver between of different groups, while there were significant differences between groups in body weight gain of the groups. Highly significant differences existed between groups, concerning the daily feed intake, relative weight of kidney, glucose serum, cholesterol and triglycerides.