Psyllium seeds are a major source of soluble and viscous gel-forming fiber that has been associated with many health benefits such as lowering blood glucose and cholesterol and improving digestive disorders. The study aimed to evaluate the effect of feeding with psyllium seeds powder in obese rats fed on a high-fat. The results indicated that feeding with a high-fat diet caused an increase in the final weight, BWG (%), feed intake, serum glucose, serum lipids, kidney and liver functions as compared negative control group (G1) as result of obesity. On the other hand, feeding with psyllium seeds powder on obese rats fed a high-fat diet significantly reduced in the final weight, BWG, feed intake and FER. Main while, serum glucose was reduced in all treated groups (G3, G4, G5 and G6) as compared with the positive control group. This reduction was ranged from 84.25 mg/dl(G3) to 76.00 mg/dl (G6) furthermore, G6 that fed on 12% psyllium seeds powder whose showed the highest decreased on TC,TG and LDL by means 134,98.25,60.35 mg/dl, respectively and highest increased in HDL by mean 55.25 mg /dl as compared with the positive control group (G2) followed by G5. All treated groups referred to improvement in kidney and liver functions as compared with the positive control group. In conclusion, data of the present study recommended that the daily consumption of psyllium seed obese patient's diets could improve serum glucose and lipid profile and promote weight loss.