Exposure to CCl₄ may increase the production of reactive oxygen species, which results in the damage of tissues, especially the liver. The purpose of this study is to evaluate the effects of whey protein (wp), α-lactalbumin(α- la )and β-lactoglobulin (β-lg) (100mg each) on the superoxide dismutase (SOD) status, liver and kidney functions after acute CCl₄ exposure in the rats. The results showed that with the feeding on WP, α-la and β-lg, the SOD level displayed a significant increase after acute exposure to CCl₄. The parameters of liver functions showed a significant decrease in the transferases (ALT, AST & GGT) and a significant increase in protein synthesis (TP & albumin). The kidney functions parameters (uric acid & createinine) are significantly reduced when WP, α-la and β-lg were administered. These results suggest that WP and its fractions should be a precursor agent to promote the production of SOD that enhance the antioxidant capacity and improve the liver and kidney functions.