This research was carried out to study the bioactive compounds which found in red beet roots as antimicrobial activity and as anticancers in the lung and breast . Red beet roots were used and a set of technological treatments (drying, blanching than drying and freeze-drying) were carried out to choose the most appropriate treatments to reduce the loss of bioactive substances. Chemical properties, minerals and active substances were estimated for each of the previous treatments.   Results showed that the drying treatment of red beet roots exhibited the lowest rates of loss in chemical composition and active substances.   Methanolic extract of fresh red beet roots and other treatments of red beet roots as antimicrobial activity of some types of bacteria, fungi and yeasts, and the results showed positive effects on bacteria and yeast.   Also, the effect of the methanolic extract of fresh beet roots and other treatment of  red beet roots were examined as anticancers on the carcinogenic cells of the lung and breast.The results showed that the IC₅₀ obtained from fresh and dried red beet roots extract were 352, 227, 242 and 321 µg/ml on both of  breast and lung cancer cells, respectively. Therefore, the study recommends that red beet roots could be added in many foodstuffs to reduce the incidence of cancer.