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125183

ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA

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Last updated: 04 Jan 2025

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Abstract

      Strain AK71, isolated from Egyptian fermented milk named Zabady, and identified as Enterococcus faecalis, produces a 4000 Da bacteriocin-like compound with inhibitory activity against Staphylococcus aureus 91161, Enterococcus faecalis 90519 and Serratia marcescens 98027. Bacteriocin AK71-like compound is inactivated by proteinase K but not when treated with catalase and trypsin. No change in activity was recorded after either 30 min or 2 h at pH values between 3.0 and 8.0, and after treatment at 100°C for 30 min. Activity was lost after treatment at 121°C for 20 min. The mode of action is bacteriostatic. The highest level of bacteriocin activity (51200 AU/ml) was recorded when cells were grown in MRS broth at 30°C for 12 h. Bacteriocin AK 71 shows activity towards Gram-negative bacteria making it different from  most bacteriocins described for Enterococcus spp.

DOI

10.21608/jfds.2008.125183

Keywords

Bacteriocin AK71, Enterococcus faecalis

Authors

First Name

M.

Last Name

Metwally

MiddleName

M.

Affiliation

Dairy Science and Technology Dept,, Fac. Agric.,Cairo Univ., Cairo, Egypt.

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First Name

A.

Last Name

Abdel-Fattah

MiddleName

M.

Affiliation

Dairy Science and Technology Dept,, Fac. Agric.,Cairo Univ., Cairo, Egypt.

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Orcid

-

First Name

S.

Last Name

Abdel-Ghany

MiddleName

-

Affiliation

Dairy Microbiology Lab, Food Industries and Nutrition Division, NRC, Cairo, Egypt.

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-

Orcid

-

First Name

A.

Last Name

Geis

MiddleName

-

Affiliation

Federal Dairy Research Centre, Institute for Microbiology, Kiel, Germany.

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City

-

Orcid

-

First Name

K.

Last Name

Heller

MiddleName

J.

Affiliation

Federal Dairy Research Centre, Institute for Microbiology, Kiel, Germany.

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-

City

-

Orcid

-

First Name

A.

Last Name

Okda

MiddleName

Y.

Affiliation

Dairy Microbiology Lab, Food Industries and Nutrition Division, NRC, Cairo, Egypt.

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-

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Volume

33

Article Issue

11

Related Issue

18736

Issue Date

2008-11-01

Receive Date

2020-11-24

Publish Date

2008-11-01

Page Start

7,997

Page End

8,020

Print ISSN

2090-3650

Online ISSN

2090-3731

Article File

JFDS_Volume 33_Issue 11_Pages 7997-8020.pdf

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Link

https://jfds.journals.ekb.eg/article_125183.html

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https://jfds.journals.ekb.eg/service?article_code=125183

Order

9

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Original Article

Type Code

886

Publication Type

Journal

Publication Title

Journal of Food and Dairy Sciences

Publication Link

https://jfds.journals.ekb.eg/

MainTitle

ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF 4 KDA BACTERIOCIN, PRODUCED BY Enterococcus faecalis AK71, WITH ACTIVITY AGAINST GRAM- POSITIVE AND GRAM-NEGATIVE BACTERIA

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Article

Created At

22 Jan 2023