The changes in amino acids in colostrum and transient milk of buffaloes and cows colostrum in the first five days and after 14 days of parturition were followed. Colostrum and milk samples were collected at calving, 6, 12, 24, 48, 72, 96, 120 h and after 14 days of parturition. Amino acid concentrations (%) in samples were determined in milk protein wit an automatic amino acid analyzer. Results showed that at calving, the concentration of leucine, proline, cysteine and tyrosine was
significantly higher in buffaloes than cows colostrum while the concentration of serine, glycine, alanine and arginine had the opposite trend. In post-partum period, among amino acids significant increases were observed in methionine and proline and significant decreases were recorded in threonine and serine of both colostrums. Arginine of  both colostrums, glycine and alainine of buffaloes colostrum and cysteine and tyrosine of cows colostrum fluctuated within the times of study, while leucine, lysine, glutamic of buffaloes and aspartic of cows colostrum revealed no significant differences during the experimental period. On the fifth day, the buffaloes milk was characterized by a significant higher isoleucine, histidine, proline, cysteine, tyrosine and arginine; and a significant lower valine, glycine and alanine concentrations as compared with cows milk.