Diabetic disease has considered a major worldwide public health problem , has bad complications and also has different etiological agents such as environmentally – physiologically – immunologically – genetically reasons . It is found that the genetic variants at ABO Locus affect diabetes risk and thus ABO blood group phenotypes may be associated with diabetic incidence . So ,the present study aims to determine frequency of blood groups ABO and rhesus factor Rh phenotypes in female diabetic patients .This research includes number of diabetic populations samples which are collected from some  clinical laboratories , hospitals in some regions of Aljabal  Alakhdar in Libya . By current criteria for diagnosis and taking patient history , the diabetes could be classified into three types (the first type (1)  , the second type( 2) and the third type (3)). Then, ABO group techniques  were worked by agglutination test and statistical analysis was carried out  by split plot design .The findings in this research have supported the hypothesis that diabetes and blood group types are associated. Also, it could be suggested that the factor Rh which is on red blood cells may have a role to some extent in determine the type of diabetes in related to ABO groups .