Diabetic disease has considered a major worldwide public health problem , has bad complications and also has  different etiological agents such as environmentally , physiologically , immunologically and genetically reasons . It is found that the genetic variants at ABO Locus affect diabetes risk and thus ABO blood group phenotypes may be associated with diabetic incidence. So, this study aims to determine the frequency of blood groups (ABO) and rhesus factor (Rh) in male diabetic patients. Samples of diabetic populations are collected from some clinical laboratories, hospitals in some regions of Aljabal Alakhdar  in Libya. By current criteria for diagnosis and taking patient history, it could be classified the diabetes in male individuals into (two typesthe first type (1) and the second type ( 2) ). Then, ABO group technique was carried out  by using agglutination test and statistical analysis was done by split plot design. The  findings in this research have supported the hypothesis that diabetes and blood group types are interrelated. Also it could be suggested that the Rh factor may have a role to some extent in determine the type of diabetes related to ABO groups.