The present study was designed to investigate the effect of some sources of dietary fibers (wheat bran, pectin and soybean hulls) at a level of 10% on the blood cholesterol level and serum transaminase enzymes, as well as, body weight, food intake, food efficiency ratio (FER) and some organs weight in hypercholesterolemic rats. The supplementation of basal diet with dietary fibers led to significant increase of final body weight, body weight gain (%), food intake and FER. Hypercholesterolemic rats had a higher level of total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C) and total cholesterol/high density lipoprotein-cholesterol (TC/HDL-C) and lower level of HDL-C comparing with normal rats. The addition of dietary fibers in all forms to basal diet improved the previous parameters and they became in the normal range. The feeding of hypercholesterolemic rats on diet containing dietary fibers decreased the liver content of total lipids, phospholipids, cholesterol and triglycerides (TG) to the level, where no significant difference was noticed with normal group. Also, the results revealed that, fecal output, fecal lipid and fecal cholesterol excretion were increased with supplementation of basal diet with dietary fibers. This supplementation improved serum transaminase enzymes especially alanine-aminotransferase enzyme (AST). So, it can be recommended that, using dietary fibers (wheat bran, pectin and soybean hulls) at the level of 10% had a pronounced effect in reducing cholesterol levels.