The present investigation was undertaken to study the preparation of gluten free biscuits from multi grains flour blend consists of rice (80%), maize (5%), and roasted chickpea (10%) as well as mashed roasted sweet potato (5%). These ratios of blend are constant and considered as control, while the Rice flour in other treatments was replacement with different ratio of quinoa flour (5, 10, 15 and 20%).  The baked biscuits were evaluated for proximate composition, amino acid pattern, sensory attributes and calcium (Ca), iron (Fe), potassium (K) and zink (Zn) contents. The results showed that the substitution of rice flour with quinoa flour improved the biscuits nutritional value where, the protein, ash, Fe, Zn, Ca and K contents were increased. Likewise, amino acids values were increment except tyrosine and methionine, which didn't affect by the replacement process, whereas the values of proline and valine were decreased. All samples of baked biscuits were acceptable and no differences were detected in the sensory characteristics on the hedonic scale in terms of, colour, texture, odor, taste, chewing and overall acceptability.