This investigation was aimed to studythe effect of iron deficiency anemia rats feeding on red beetroot juice.  The present investigation demonstrated that red beetroot  could be  comprises of mixes with the capacity to explicitly animate duodenal iron a ingestion. Red beetroot  was analyzed for chemical compositions ( ash, crude fats, crude proteins, crude fiber, carbohydrates, phenolic and flavonoids compounds). At feeding experiment was conducted on iron deficiency anemia rats fed on red beetroot . The outcomes demonstrated that iron-inadequacy caused many turn around impacts mirrored the noteworthy decrease of HCT, Hb, RBC, WBC, MCV and MCHC. On contrary, administration of ferrous sulphate (FeSO₄) and red beetroot juice induced a significant increase in Hb, HCT, RBC, WBC,MCV and MCHC values  of  serum iron profile.