Cardiovascular disease is one of the major causes of death worldwide. Diet is the most editable factor in the prevention of this disease. Green tea beverage (GTB) as a water extract was prepared and biologically evaluated for its anti-atherogenic potential in Wister rats. GTB prepared in the present study showed high antioxidant activity as obtained in radical scavenging activity (86%), Ferric reducing antioxidant power (2.566) and reducing power (1.687). GTB had high total phenolics (13.252 mg GAE/ml) and flavonoides (0.525mg QE/ml). In GTB-group (G3), administration of GTB led to improve serum lipid profile and oxidative stress parameters compared to the other two groups (atherogenic control group (G2) and negative control group (G1)). The histopathological investigation of the aorta, heart and liver assured these results which reflected the preventative impact role of GTB against atherosclerosis and cardiovascular diseases.