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53056

PARTIAL CHARACTERIZATION OF BACTERIOCINS PRODUCED BY TWO STRAINS OF LACTIC ACID BACTERIA, ISOLATED FROM TRADITIONAL EGYPTIAN DAIRY PRODUCTS

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Last updated: 22 Jan 2023

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Abstract

This study presents the characterization of strains of lactic acid bacteria (LAB) from traditional Egyptian dairy products. 623 isolates of lactic acid bacteria (LAB) were studied for their antimicrobial activity against taxonomically related microorganisms. Selected LAB were identified by PCR method as Enterococcus faecium IM1and Enterococcus hirae IM1. Partial purified enterocin from tested strains showed antimicrobial activity against different indicators such as: Brochothrix thermosphacta DSMZ, Carnobacterium maltaromaticum CIP103135T, Carnobacterium piscicola S4312, Enterococcus faecalis JH2, Listeria grayi CLIP12518, Listeria innocua, Listeria ivanovii ATCC,  Listeria monocytogenes and Vagococcus penaei CIP109914T but have no effect against Bacillus subtilis, Moellerella wisconsensis, Pseudomonas fluorescens, Salmonella enterica, Salmonella montevideo, Salmonella typhimurium andSerratia liquefaciens CIP103238T. The inhibitory activity was not due to hydrogen peroxide for Enterococcus hirae IM1, but strain Enterococcus faecium IM1 may excrete diverse antimicrobial compounds such as hydrogen peroxide and bacteriocins. Bacteriocins produced by isolated strainswere stable between pH 5 and 8 and stable until 100°C/ 20 min. Tested strains were free from some of virulence determinant genes. They may be useful as starter cultures and co-cultures. Consequently, the selected strains can be applied in fermented dairy foods as efficient biopreservatives cultures.

DOI

10.21608/jfds.2014.53056

Keywords

enterococci, enterocins, Listeria ssp

Authors

First Name

S.

Last Name

EL-Ghaish

MiddleName

N.

Affiliation

Kafrelsheikh University, Faculty of Agriculture, Department of Dairy Sciences, B.P. 33516, Kafrelsheikh, Egypt

Email

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City

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Orcid

-

First Name

Azza

Last Name

EL-Baz

MiddleName

M.

Affiliation

Agriculture Research Center, Animal Production Research Institute, Department of Dairy Science, Sakha, Kafr El-Sheikh, 33717, Egypt

Email

-

City

-

Orcid

-

First Name

N.

Last Name

Hwanhlem

MiddleName

-

Affiliation

Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai 90112, Thailand

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Zommara

MiddleName

A.

Affiliation

Kafrelsheikh University, Faculty of Agriculture, Department of Dairy Sciences, B.P. 33516, Kafrelsheikh, Egypt

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Ayad

MiddleName

H.

Affiliation

Alexandria University, Faculty of Agriculture - Saba Basha, Department of Food Science, P.O. Box, 21531, Bolkely-Alexandria, Egypt

Email

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City

-

Orcid

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First Name

Yvan

Last Name

Choiset

MiddleName

-

Affiliation

12UR 1268 Biopolymères Interactions Assemblages, Équipe Fonctions et Interactions des Protéines, INRA, 44316 Nantes Cedex 03, France.

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-

City

-

Orcid

-

First Name

T.

Last Name

Haertlé

MiddleName

-

Affiliation

12UR 1268 Biopolymères Interactions Assemblages, Équipe Fonctions et Interactions des Protéines, INRA, 44316 Nantes Cedex 03, France.

Email

-

City

-

Orcid

-

First Name

J.

Last Name

Chobert

MiddleName

-

Affiliation

12UR 1268 Biopolymères Interactions Assemblages, Équipe Fonctions et Interactions des Protéines, INRA, 44316 Nantes Cedex 03, France.

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-

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-

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Volume

5

Article Issue

8

Related Issue

8108

Issue Date

2014-08-01

Receive Date

2019-10-12

Publish Date

2014-08-01

Page Start

561

Page End

579

Print ISSN

2090-3650

Online ISSN

2090-3731

Article File

JFDS_Volume 5_Issue 8_Pages 561-579.pdf

PDF . 618.3kB

Link

https://jfds.journals.ekb.eg/article_53056.html

Detail API

https://jfds.journals.ekb.eg/service?article_code=53056

Order

4

Type

Original Article

Type Code

886

Publication Type

Journal

Publication Title

Journal of Food and Dairy Sciences

Publication Link

https://jfds.journals.ekb.eg/

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Article

Created At

22 Jan 2023