This investigation was carried out to study the hypoglycemic effects of Chromium picolinate and Magnesium sulfate on streptozotocin induced diabetic rats. Chromium at levels of 8 and 10ug/ml, Magnesium at level 10 and 12mg/ml. Furthermore, mixture of (chromium 8u/ml + Magnesium10mg) and (chromium10u/ml + Magnesium12mg/ml) administered to diabetic rats for six weeks. Blood glucose and, body weight gain of rats were determined. Also, total cholesterol, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and triglycerides were determined in the serum of the rats. As well as the activities of serum aspartate transaminase (AST) and alanine transaminase (ALT) were determined. The results indicated that Chromium picolinate and Magnesium sulfate led to a significant reduction in weight gain and blood glucose of diabetic rats. In addition, total cholesterol, LDL-C, triglycerides, creatinine and urea in serum decreased while HDL-C increased after administration Chromium picolinate and Magnesium sulfate. The activities of hepatic markers were significantly decreased in GPT, but GOT no change elevated in diabetic rats as compared to control rats. Finally, it can be concluded that, using different levels of Chromium picolinate, Magnesium sulfate and mixture of them have pronounced effect for lowering blood glucose and cholesterol levels of the serum in experimental diabetic rats.