It is important to know the antioxidant content and their efficacy in foods, for preservation or protection against oxidative damage, to avoid deleterious changes and loss of commercial and nutritional value. Antioxidants are the compounds , which combat the free radical by intervening it any one of the free radical mediated oxidative process. The role of ethanolic  extracted kernel of Balanites aegyptiaca (B. aegyptiaca) on development hepatocarcinoma and CCL4 effect as an antioxidant and antimicrobial were identified in this study. The present study aimed to identify the potential hepatoprotective activity against hepatic injury produced by carbon tetrachloride CCL₄ in rats and to acquire information about the health aspects of Balanites aegyptiaca.The DPPH radical scavenging activity assay (ROS )activity of Balanites aegyptiaca (50 ; 100 ; 200 mg/mL) was determined against standard BHT. TheScavenging effect of kernel ethanolic extract of Balanites aegyptiaca (EB_i ) on the DPPH radical decreased in order of EB 200 >EB 100 >EB 50 at all concentrations (200 , 100 and 50 mg/mL) respectively, this actually occurred linearity with increasing concentrations and due to increase flavonoids that has apotent antioxidant activity and its strongscavengers of free radicals.The antimicrobial activity of the kernel of Balanites aegyptiaca (EB_i) and different extracts  doses well diffusion method are determined by using inhibition zone against Psedomonas  aeruginosa, Staphylococcus  aureus and Streptococcus  faecalis. The inhibition zone for Psedomonas  aeruginosawas 9 mm at dose EB 50 . Dose EB200 was more susceptible to inhibit bacterial activity of Pseudomonas  aeruginosa, Staphylococcus  aureus and Streptococcus  faecalis were found to EB more sensitive than other doses of EB100 and EB50. The minimal concentration of ethenolic extract of minimum inhibitory concentration (MIC₉₀) as more susceptible to inhibit 90% of  Staphylococcus  areas with lower concentrate 132 µl/ml while , Streptococcus  faecalis was need for higher concentrate from extract at 190 µl/ml. The biological study was carried on albino rats Liver injury induced by an oral administration of 20%  Carbon tetrachloride (CCL₄) which was suspended in corn oil and then injected orally (1 ml/kg body weight), twice a week, for 4 weeks. Then rats were grouping into 4 groups and dosing by 200 mg of Balanites aegyptiaca  and  compared with 50 mg per rat on Silymarin. Antioxidant and biochemical determination were recorded. B. aegyptiaca extracts inhibited oxidative of CCL4 effect was significant decrease in treated animals for all different four groups where  a significant reduction (P<0.05) in the serum at liver function (ALT and AST) levels were occurring among 200 mg fed group (3) of and also among group 4 which dosages on Silymarin treated when comparison with hepatocarcinoma group 2 of CCL₄ .Also there was definitly improving liver function and ALP (alkaline phosphatase), indicated that EB 200 had a significant role to recovery of some hepatocarcinoma symptoms, which occurred by CCL₄ treated and near to Silymarin in its activity.  Also the  EB extract at 200 mg it  is coming in the second order after Silymarin effective when compared to antioxidative role by Glutathione reductase (GSSG-R)  of Silymarin. Histopathological investigation had shown that, the  kernel extract for EB 200 mg and 50 mg Silymarin were responsible to eliminate and recover treated groups by CCL₄ (hepatocarcinogenic agent) from inflammation. It could be concluded that, the EB 200 can provide a considerable ratio of antioxidants and antinflammatory were effectively upon treated CCL₄ and other carcinogenic agents. This result gives a new insight on beneficial effect of kernel B. aegyptiaca extract  in antioxidant  and antimicrobial as well as against hepatocarcinogenic agents such as CCL₄ effect.