Hyperlipidemia is defined as increase in the lipid content in blood. This study aimed to evaluate the effect of supplementation of hyperlipidemic diet with different levels of lemon peels on lipid profile and other biochemical parameters of hyperlipidemic rats. Results showed that rats fed diet with high cholesterol exhibited significant increasing in body weight, liver weight, total serum cholesterol, triglycerides, low density lipoprotein-cholesterol (LDL-C), very low density lipoprotein-cholesterol (VLDL-C) and significant decreasing in high density lipoproteins-cholesterol (HDL-C) Treatment with lemon peels significantly decreased body weight, total serum cholesterol, triglycerides, low density lipoprotein(LDL-C), very low density lipoprotein (VLDL-C) and increased the level of high density lipoproteins(HDL-C). The level 10% recorded the best results followed by 5 and 2.5% Plasma calcium was the high affected by increasing the level of lemon peels. Finally, it can be concluded that lemon peels improved lipid profile and the other biochemical parameters without increasing the using level above 10%