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253122

Ethylenediamine tetra acetic acid (EDTA) enhances the antitumor efficacy of cisplatin against human breast cancer cells in vitro

Article

Last updated: 22 Jan 2023

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Subjects

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Tags

Natural/synthetic agents in anti-cancer therapy

Abstract

Background: Ethylenediamine tetra acetic acid (EDTA) is used in several biomedical applications. Aim: The aim of this study was to investigate the effect of EDTA treatment on anticancer efficacy of cisplatin (Cis) against human breast cancer (MCF-7) cells in vitro. Materials and Methods:MCF-7 cells were treated either with Cis, EDTA, or their combination for 24h in vitro. The percentages (%) of the inhibitory, and the median inhibitory concentration (IC50) of EDTA were determined by MTT assay. The % of Cis and EDTA on early and late apoptosis, necrosis, and cell cycle of MCF-7 were assessed by flow cytometry. Results: Our data showed slight antitumor effects for EDTA in vitro. However, Cis/EDTA treatment increased the antitumor efficacy of Cis as evidenced by increasing IC50, and the percentage of MCF-7 mortality. Cis/EDTA co-treatment also increased the % of apoptotic and necrotic MCF-7 cells post 24 h of treatment (26.57 and 16.28%, respectively). Furthermore, this co-treatment arrested MCF-7 cell cycle at G0 phase (32.8%) and G2/M phase (30.25%). Conclusion: Co-treatment of EDTA  with Cis increased the anticancer efficacy  of Cis.

DOI

10.21608/jcbr.2022.96763.1233

Keywords

EDTA, Cisplatin, MCF-7 cells, in Vitro, Anti-tumor

Authors

First Name

Sabry

Last Name

El-Naggar

MiddleName

A.

Affiliation

Zoology Department, Faculty of Science, Tanta University, Tanta 3111, Egypt

Email

sabry_elnaggar@yahoo.com

City

Tanta

Orcid

0000-0002-7018-9350

First Name

Yousry

Last Name

El-Bolkiny

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science, Tanta University, Egypt

Email

bolkiny61@yahoo.com

City

Tanta

Orcid

0000-0003-4001-6034

First Name

Fatheya

Last Name

Ibrahim

MiddleName

Makboul

Affiliation

Zoology, Faculty of Science,Suez canal University

Email

fmakbou27@gmail.com

City

Suez

Orcid

0000-0002-3194-5854

Volume

6

Article Issue

2

Related Issue

36020

Issue Date

2022-06-01

Receive Date

2021-09-19

Publish Date

2022-06-01

Page Start

23

Page End

29

Print ISSN

2682-261X

Online ISSN

2682-2628

Article File

JCBR_Volume 6_Issue 2_Pages 23-29.pdf

PDF . 1.0MB

Link

https://jcbr.journals.ekb.eg/article_253122.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=253122

Order

3

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023